1) Oxygen requirement: Bacteria are divided into four groups according to oxygen requirement: 1. After incubation, the bacteria appear to be growing throughout the tube. Determine the effects of several chemical and physical factors on the growth of different microorganisms; 2. Oxygen requirement. Growth factors are organic compounds such as amino acids , purines , pyrimidines , and vitamins that a cell must have for growth but cannot synthesize itself. Transformation happens very slowly, but … Choose an appropriate strategy for this experiment. Classify different bacterial species according to their response to various temperature, pH, oxygen and salinity/osmolarity. Transformation is the introduction of foreign DNA into a bacterial cell. Factors affecting bacterial growth Factors affecting bacterial growth are: 1. If you want to do a science fair project about germs, you have to add a variable, or something that changes in the experiment. Bacteria grow only in the presence of oxygen, called: strict aerobes. Factors affecting bacterial growth: _____ (Fig 6.17) - formation of endospores in Bacillus, Clostridium and a few other organisms in response to harsh conditions. PH 4. Bacteria can be grouped according to their energy source as phototrophs or chemotrophs. Before we go over these factors we must however understand the bacterial growth curve. Just growing bacteria in a Petri dish is not a science fair experiment. The survival and growth of a microbe is a function of a myriad of different activities: nutrient assimilation, catabolism, and the synthesis of living cytoplasm and all cellular structures. 2. Temperature requirement. What are environmental factors that affect microbial growth?-atmospheric gases-temperature-pH-osmotic pressure ... A tube of fluid thioglycliate medium is inoculated with a bacterial isolate. Osmotic pressure (salt tolerance). EXPERIMENT 6. As E.coli grows in the intestines its optimal temperature is about 37 degrees c.pHE. TemperatureLow temperatures slow down while temperatures higher than 40 degrees c will denature the bacteria, an optimal temperature is needed for optimal growth. There are many factors that affect bacterial growth. The bacterial growth curve represents the number of live cells in a bacterial population over a period of time. 2. Laboratory Exercise 14 PHYSICAL AND CHEMICAL FACTORS AFFECTING GROWTH Objectives: At the end of the experiment, the student must be able to: 1. The Effect of Alkalines and Acids on Bacterial Growth Biology Projects,Science Experiments Project Ideas, Science Experiment Ideas, Model Topics, Exhibition Ideas, Expo Activities for CBSE ICSE for Kids and also for Middle school, Elementary School for class 5th Grade,6th,7th,8th,9th 10th,11th, 12th Grade and High School , MSC and College Students. Maintaining work equipment and the physical state of a building is important, but there are potential threats lurking in a company’s infrastructure that are too small for the naked eye to see. Occurs in stationary phase, when nutrients are limiting, and/or wastes have accumulated. Endospores are dormant, and are resistant to heat, radiation, drying, and many chemicals. Bacterial transformation is a common technique in molecular biology that every student of the life sciences should be familiar with. Factors Affecting Microbial Growth The key to a productive staff is creating and maintaining a safe and healthy work environment. All these various activities are performed by specific enzymes. Growth factors. coli can survive in stomach acid of pH 2 but optimal pH for the growth of E.Coli is… FACTORS INFLUENCING THE GROWTH OF MICROBES . A small group of cells are placed in a nutrient rich medium that allows them to synthesize proteins and other molecules necessary for replication. 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